Diol HPLC Columns-Hydrophilic Interaction & Normal Phase

Diol HPLC Column

  • Unique Stationary Phase Bonding

  • High Column Efficiency: Theoretical Plate Number >80000/m

  • Wide pH range: Suitable for pH 2.0-8.0

  • Extended Lifespan

  • Ultra-Pure Fully Porous Spherical Silica Gel Purity >99.999%

  • Good Peak Shape Symmetry: Tailing Factor Controlled within 0.95-1.05

  • Complete Range of Stationary Phases, Offering a Wide Spectrum of Selectivity

  • Reasonable Price

  • Best Service

Description

Product Introduction

Diol Column is based on ultra-pure porous spherical silica bonded with 1,2-dihydroxypropyl functional group silica.Diol is used in normal phase mostly, suitable for separation of peptides, proteins, polar molecules, and organic acids and its polymers.Like bare silica, Diol has the ability to form hydrogen bonds and is capable of separating structure isomers. Since most of its surface is covered with organic functions, Diol absorbs less water, which leads to more reproducible activity. It is also the sorbent of choice when working in normal phase in the presence of water. It has a different selectivity than bare silica gel, and slight modification in the composition of solvent mixture may be necessary to obtain a similar retention.Diol column is more stable than traditional normal phase columns, such as NH2, SiO2. Compared with NH2/SiO2 column,Diol column is not sensitive to water. Diol column can also be used in reversed phase analysis.


Features

1. More stable than traditional normal phase columns, such as Silica, Amine.

2. Can be used in reversed phase analysis.

3. Similar polarity to Amine.

4. Good selectivity without excessive retention.

5. Improved peak shape compared to bare silica.


Specifications

pH Range2.0-8.0
Particle Size3μm, 5μm, 10μm
Surface Area(m2/g)320(120Å)
Carbon Loading(%)2.5(120Å)
USP ListL20
EndcappedNo


Ordering Information—Diol

Particle SizeID(mm)Column Length (mm)






305075100150200250300

3μm

120 Å

2.1YZHDI2130-3YZHDI2150-3YZHDI2175-3YZHDI21100-3YZHDI21150-3YZHDI21200-3YZHDI21250-3-
3.0YZHDI3030-3YZHDI3050-3YZHDI3075-3YZHDI30100-3YZHDI30150-3YZHDI30200-3YZHDI30250-3-
4.0YZHDI4030-3YZHDI4050-3YZHDI4075-3YZHDI40100-3YZHDI40150-3YZHDI40200-3YZHDI40250-3-
4.6YZHDI4630-3YZHDI4650-3YZHDI4675-3YZHDI46100-3YZHDI46150-3YZHDI46200-3YZHDI46250-3-

5μm

120 Å

2.1YZHDI2130-5YZHDI2150-5YZHDI2175-5YZHDI21100-5YZHDI21150-5YZHDI21200-5YZHDI21250-5-
3.0YZHDI3030-5YZHDI3050-5YZHDI3075-5YZHDI30100-5YZHDI30150-5YZHDI30200-5YZHDI30250-5-
4.0YZHDI4030-5YZHDI4050-5YZHDI4075-5YZHDI40100-5YZHDI40150-5YZHDI40200-5YZHDI40250-5-
4.6YZHDI4630-5YZHDI4650-5YZHDI4675-5YZHDI46100-5YZHDI46150-5YZHDI46200-5YZHDI46250-5-

10μm

120 Å

4.0----YZHDI40150-10YZHDI40200-10YZHDI40250-10YZHDI40300-10
4.6----YZHDI46150-10YZHDI46200-10YZHDI46250-10YZHDI46300-10

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